primary rat dermal fibroblasts  (PELOBIOTECH GmbH)

Journal: International Dental Journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Effects of different Dicliptera chinensis polysaccharide (DCP) concentrations on the viability of rat dermal fibroblasts (RDFs). CCK8 assay was used to examine the role of DCP in RDF cell lines. Cells were inoculated into 96-well plates and exposed to the specified concentrations of DCP (50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, and 1.6 mg/mL) for 24 hours. The result shown is the standard deviation average of 3 independent experiments. The significance was analyzed using one-way analysis of variance with Dunnett-t test to compare the means between the control and experimental groups (* P < .05, ⁎⁎ P < .01, ⁎⁎⁎ P < .001 and blank group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: CCK-8 Assay, Standard Deviation, Control

Journal: International Dental Journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Effect of Dicliptera chinensis polysaccharide (DCP) on radiation-induced apoptosis of rat dermal fibroblasts (RDFs). (A) RDFs were cultured in 100 μg/mL and 200 μg/mL DCP with experimental concentrations for 24 hours and then irradiated with 8 GY X-rays. The cells were then stained with Annexin V- Fluorescein Isothiocyanate and propium iodide and detected by flow cytometry. (B) The result shown in B is the standard deviation average of 3 independent experiments. The significance was determined using one-way analysis of variance (* P < .05, ⁎⁎ P < .01, ⁎⁎⁎ P < .001 and ionising radiation [IR] group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: Cell Culture, Irradiation, Staining, Flow Cytometry, Standard Deviation

Journal: Scientific Reports

Article Title: The monoclonal antibody EPR1614Y against the stem cell biomarker keratin K15 lacks specificity and reacts with other keratins

doi: 10.1038/s41598-018-38163-5

Figure Lengend Snippet: Organotypic cultures (OTCs) of keratinocyte cell lines. HaCaT ( A ), N/Tert-1 ( B ), KEB-11 ( C ) and NEB-1 ( D ) cells were co-cultured with primary dermal fibroblasts in OTCs for 7 and 9 days, followed by paraffin embedding and sectioning. Images of H&E-stained specimens were acquired using Leica Epi DM5000B or Nikon Eclipse 80i microscopes; scale bar = 200 μm.

Article Snippet: HaCaT, N/Tert-1, KEB-11 and NEB-1 keratinocytes were seeded on a human primary dermal fibroblast containing disc of rat-tail collagen type I (Corning, UK) in a Millipore ® Millicell ® (Sigma-Aldrich, UK).

Techniques: Cell Culture, Staining

Journal: PLoS ONE

Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor

doi: 10.1371/journal.pone.0043155

Figure Lengend Snippet: A , Rat dermal fibroblasts (RDF) and B , human dermal fibroblasts (HDF) were treated with 10 ng/ml hLIF, hOSM, mOSM or rOSM for 15 min. The phosphorylation levels of STAT1, STAT3, ERK1/2 as well as STAT5, p38 and Akt were detected via Western blot analysis. The blots were stripped and reprobed with antibodies recognizing the proteins irrespective of their activation status. Additionally an α-tubulin loading control was included. Phosphorylation intensities were quantified by chemiluminescence analysis and normalized to tubulin. Activation determined for rOSM was set to 100%. Shown are the means (n = 3) with standard error of mean (SEM). * p<0.05, ** p<0.01, *** p<0.001 untreated vs. cytokine-treated sample.

Article Snippet: Primary rat dermal fibroblasts were obtained from PELOBiotech (Martinsried, Germany).

Techniques: Phospho-proteomics, Western Blot, Activation Assay, Control